Modulation of γ-Irradiation Induced ROS Responses by Engineered Fullerenes in Human Epidermal Keratinocytes
نویسندگان
چکیده
High-dose ionizing irradiations can cause acute tissue and organ damage, especially severe cutaneous lesions. These cutaneous injuries often result in further complications and incur high mortality. Therefore, it is critical to develop effective therapeutic approaches for acute high-dose ionizing irradiation injury. Intracellular reactive oxygen species (ROS) have been implicated in mediating irradiation-induced cell damage. In the present study, we investigated the protective effect of engineered fullerene nanoparticles on γ irradiation-induced intracellular ROS levels in human epidermal keratinocytes (HEK). Cells were exposed to water-soluble fullerene derivatives at concentrations of 25, 50, and 100 μg/ml. Exposure of HEK cells to high-dose gamma (γ) irradiation (10 and 17 Gy) resulted in a significant increase in cellular ROS levels. The observed γ-irradiation induced ROS response was notably attenuated by modified-fullerenes, CD-C60, tris-C60 and hexa-C60. Our results point to the potential use of fullerene derivatives as novel therapeutic agents for protection against high-dose irradiation generated ROS-induced cellular damage.
منابع مشابه
Possible protective effect of naringin (a citrus bioflavonoid) against kidney injury induced by γ-irradiation and /or iron overload in male rats
Background: the supplementation of natural antioxidants such plant flavonoids may counteract the deleterious actions of gamma-irradiation and iron overload. Material and Methods: the protective effect of naringin (NIN) against kidney injury induced by γ-irradiation (IRR) (6.5 Gy) and/or iron dextran (ID) (50 mg/kg. b. w) has been studied in rats given 80 mg/kg b.w of NIN for 14 days before, and...
متن کاملBNIP3 upregulation via stimulation of ERK and JNK activity is required for the protection of keratinocytes from UVB-induced apoptosis
The human skin has an important role in barrier function. Ultraviolet rays (UV) from sunlight exposure can cause cell apoptosis in the skin epidermis, resulting in the disruption of the barrier. Previously, we have demonstrated that BNIP3 stimulates autophagy in epidermal keratinocytes and has a protective effect in these cells upon UVB irradiation. In this study, we found that the accumulation...
متن کاملA novel mechanism of γ-irradiation-induced IL-6 production mediated by P2Y11 receptor in epidermal keratinocytes.
Skin inflammation is caused by excessive production of cytokines and chemokines in response to an external stimulus, such as radiation, but the mechanisms involved are not completely understood. Here, we report a novel mechanism of γ-irradiation-induced IL-6 production mediated by P2Y11 receptors in epidermal cells. After irradiation of HaCaT cells derived from human epidermal keratinocytes wit...
متن کاملIsolation and in vitro cultivation of human skin Keratinocytes and preparation of epidermal sheet
متن کامل
Protective Effects of Lindera coreana on UVB-induced Oxidative Stress in Human HaCaT Keratinocytes
The present study was designed to investigate the protective effect of ethanol extracts from Lindera coreana leaves (LCE) on UVB-induced oxidative stress in HaCaT keratinocytes. The HaCaT cells were pretreated with LCE for 24 h and then exposed to UVB (20 mJ/cm2) for 2 h. UVB significantly decreased the cell viability (p
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2008